Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
نویسندگان
چکیده
A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.
منابع مشابه
Partial characterization of nucleoids and nucleoid-plasmid complexes from Salmonella typhimurium.
Nucleoids from Salmonella typhimurium strain LT2 consist of supercoiled deoxyribonucleic acid structures that are ribonuclease labile sedimenting at 1,700S. More than 90% of the covalently closed circular deoxyribonucleic acid of a cryptic plasmid harbored by this strain cosediments with the host's 1,700S nucleoids.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 119 3 شماره
صفحات -
تاریخ انتشار 1974